S1013-57C

Siglec 1 (Sialic acid binding Ig-like lectin 1, sialoadhesin, Siglec 1, CD169, CD169 antigen, Sialoadhesin, Sialoadhesin precursor, Siglec 1)

Size

£869.00

SKU:: S1013-57C
Purity:: Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/1mg (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Ser20-Gln1641 of human Siglec-1 expressed in NS0 cells
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml using SH‑SY5Y human neuroblastoma cell line and human lymph node Neutralization: 1.5-7.5ug/ml (ND50) in the presence of 5ug/ml recombinant human Siglec-1/CD169 Fc Chimera. Optimal dilutions to be determined by the researcher. Storage and Stablility: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.