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S1013-37

Sialoprotein, Bone, Carbohydrate Deficient (Carbohydrate Deficient Bone Sialoprotein, CD-BSP)

Size

£384.00

SKU:
S1013-37
Purity:
Purified by Protein G affinity chromatography from cell supernatant.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rat
Immunogen:
Recombinant protein corresponding to aa108-122 from human CD-BSP
Clone:
IDBSP5
Format:
Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 10ul or 100ul sterile ddH2O.
Extra Details:
Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 1:250 Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher. Matched Pair: Suitable for use as the capture or solid phase antibody. Matched to S1013-36A as the detection or conjugate (label) antibody. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice