S1013-36A
Sialoprotein, Bone (BSP, Bone Sialoprotein 2, BSP2, Cell-binding Sialoprotein, Integrin-binding Sialoprotein)
Size
£351.00
- SKU:
- S1013-36A
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human Bone Sialoprotein (Bone extract).
- Clone:
- ID1.2
- Format:
- Supplied as a lyophilized powder in 50mM Tris, pH 7.2. Reconstitute with 10ul or 100ul sterile ddH2O.
- Extra Details:
- Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, RIA and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:400-1:2000 Immunohistochemistry (Frozen): 1:100 Immunocytochemistry: 1:200 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice