S1013-28
SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 Containing Protein Tyrosine Phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)
Size
£544.00
- SKU:
- S1013-28
- Molecular Weight:
- 34.3
- Purity:
- ≥ 95% by SDS PAGE. Endotoxin: ≤1EU/ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT, 20% glycerol
- Extra Details:
- The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). Additional amino acid (Met) is attached at N-terminus. Recombinant protein corresponding to aa243-541 from human, expressed in E. coli. Accession: NP_002822 Amino Acid Sequence: MGFWEEFESL/ QKQEVKNLHQ/ RLEGQRPENK/ GKNRYKNILP/ FDHSRVILQG/ RDSNIPGSDY/ INANYIKNQL/ LGPDENAKTY/ IASQGCLEAT/ VNDFWQMAWQ/ ENSRVIVMTT/ REVEKGRNKC/ VPYWPEVGMQ/ RAYGPYSVTN/ CGEHDTTEYK/ LRTLQVSPLD/ NGDLIREIWH/ YQYLSWPDHG/ VPSEPGGVLS/ FLDQINQRQE/ SLPHAGPIIV/ HCSAGIGRTG/ TIIVIDMLME/ NISTKGLDCD/ IDIQKTIQMV/ RAQRSGMVQT/ EAQYKFIYVA/ IAQFIETTKK/ KLEVLQSQKG/ QESEYGNITY Specific Acitivity: ≥8000unit/mg Unit Definition: One unit will hydrolyze 1nanomole of p-nitrophenyl-phosphatate (pNPP) per minute at pH 7.4, 37°C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice