S1013-26H

SHP 1, Recombinant, Human, GST-Tag (SHPTP-1, SH2 containing protein tyrosine phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)

Size

£970.00

SKU:: S1013-26H
Molecular Weight:: 95
Purity:: ≥95% by SDS-PAGE
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 75mM NaCl, 0.05% Tween 20, 50% glycerol, 2mM EDTA, 1mM DTT and 10mM glutathione.
Extra Details:: Protein tyrosine phosphatase, full length, (Gene bank accession number NM_080549) with N-terminal GST tag, expressed in an E. coli expression system. Specific Activity: 1U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay Buffer: 50mM HEPES, pH 7.4, 2mM EDTA, 3mM DTT, 100mM NaCl, 50mM pNPP. Applications: Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.