S1012-10

SETA (CIN85, Ruk, SH3KBPI)

Size

£863.00

SKU:: S1012-10
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: 6His and HA tagged fusion protein corresponding to full length rat SETA.
Clone:: 5H43 (84)
Format:: Supplied as a liquid in 0.1M Tris glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes. Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.