S1012-10-APC
SETA (CIN85, Ruk, SH3KBPI) (APC)
Size
£1,119.00
- SKU:
- S1012-10-APC
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- 6His and HA tagged fusion protein corresponding to full length rat SETA.
- Clone:
- 5H43 (84)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Extra Details:
- SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes. Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice