S1010-88B4
SHARP1 (Class E Basic Helix-loop-helix Protein 41, bHLHe41, Class B Basic Helix-loop-helix Protein 3, bHLHb3, Differentially Expressed in Chondrocytes Protein 2, hDEC2, Enhancer-of-split and Hairy-related Protein 1, SHARP-1, BHLHE41, BHLHB3, DEC2)
Size
£837.00
- SKU:
- S1010-88B4
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant corresponding to aa101-287 from human DEC2 expressed in E.coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- DEC-2 is a 54-56kD Class B member of the bHLH superfamily of proteins. It utilizes both DNA binding and protein-protein interaction to mediate E-box transcriptional repression. Physiologically, DEC-2 is proposed to be a regulator of the sleep cycle, particularly during periods of sleep deprivation. It also interacts with HIF-1 alpha neutralizing its activation of the VEGF gene during hypoxia. Human DEC-2 is 482aa in length. It contains a DNA binding motif aa45-57, an HLH region aa58-100, an Orange domain that may impart specificity to protein-protein interactions aa131-166 and a Gly/Ala-rich region aa297-431. DEC-2 forms homodimers, and heterodimers with MyoD and E-proteins. There is one potential alternate start site at Met280. Over aa101-287, there is less than 60% aa identity between human and mouse DEC-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice