S1010-87E

SGLT-2 (Sodium Glucose Transporter 2)

Size

£754.00

SKU:: S1010-87E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the 16aa sequence from the C-terminal, cytoplasmic domain 6 of human SGLT-2, conjugated to KLH. Species Sequence Homology: rabbit: ~73%; mouse: 71%.
Format:: Supplied as a liquid in PBS, 01% BSA.
Extra Details:: The kidneys play a major role in the regulation of glucose levels. Kidneys filter ~180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucose co-transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). About 3 members of SGLTs (SGLT-1-3) have been cloned and characterized from various species. Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT-1-3 genes code for protein of ~659-672 residues (calculated size of ~75kD). Both N- and C-termini are predicted to be extracellular. There is ~60-70% homology between SGLT-1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT-1 does not discriminate a-MDG, glucose, and galactose. SGLT-2/3 do not transport D-galactose efficiently. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Western Blot: 1-10ug/ml using ECL. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: S1010-87F: SGLT-2, Human, Control Peptide (Sodium Glucose Transporter 2) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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