S1010-87B

SGLT-2 (Sodium Glucose Transporter 2)

Size

£754.00

SKU:: S1010-87B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide mapping to 16aa near the N-terminal of rat SGLT-2 KLH-conjugated. Species sequence homology: mouse 86%. No significant sequence homology exists with other SGLTs.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Extra Details:: The kidneys play a major role in the regulation of glucose levels. Kidneys filter approx. 180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucose co- transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual members of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of ~659-672aa (calculated size of ~75kD). Both N and C-termini are predicted to be extracellualr. There is ~60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate among a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently. SGLT2/SLC5A2 (rat/mouse 670 aa; human 672 aa, chromosome 16p11.2) is the low affinity, high capacity Na+-glucose transporter located in the S1 segments of proximal tubules. It is ~60% identical with SGLT1. SGLT2 mediates saturable Na-dependent and phlorizin-sensitive glucose transport. Defects in SGLT2 may be associated with renal glycosuria. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: S1010-87A Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice