S1010-86A
SGLT-1 (Sodium Glucose Transporter 1) (Not for Export EU)
Size
£704.00
- SKU:
- S1010-86A
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 19aa near the extracellular domain 4 of mouse/rabbit SGLT-1, conjugated to KLH.
- Format:
- Supplied as a liquid in 0.05% sodium azide
- Extra Details:
- The kidneys play a major role in the regulation of glucose levels. Kidneys filter ~180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, sodium glucose co-transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes bya facilitated sugar transporter (GLUTs). 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual members of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of ~659-672 residues (75kD). Both N and C termini are predicted to be extracellular. There is ~60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000, ~70-77kD. ELISA: 1:10,000-1:50,000. Coat ELISA plates with control peptide (S1010-86F) at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice