Serotonin (5-Hydroxytryptamine, 5HT) (Not for Export EU)

Size

£672.00

SKU:: S1001-09A
Purity:: Serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Serotonin conjugated to BSA with paraformaldehyde.
Format:: Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS or TBS.
Extra Details:: Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: ≥1:500 Immunohistochemistry: 1:400-1:800 in PBS/0.3% Triton X-100 Cy3 technique; 1:5000-1:10,000 in PBS/0.3% Triton X-100, Biotin/Avidin-HRP Optimal dilutions to be determined by the researcher. Immunohistochemistry Application Notes: Perfusion Fixation: Fixative- 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500ml, over 20 minutes. Post Fixation: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500ml, over 20 minutes. Post Fixation: 1.5 hour at 4°C. 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1- 0.3%) may be used in conjunction with paraformaldehyde. Sections: 10um cryostat or 50um vibratome Absorption control: 5-HT/BSA 25ug/ml diluted serum. Incubation on Tissue: 16-24 hours at 4°C. Positive Control: Rat hypothalamus, raphe nuclei, spinal cord Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile TBS or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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