S1000-89A-BIOTIN

SERPINB9 (Serine/Cysteine Proteinase Inhibitor, Clade B, Member 9, Serpin B9, PI9, Cytoplasmic Antiproteinase 3, CAP-3, CAP3) (Biotin)

Size

£940.00

SKU:: S1000-89A-BIOTIN
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant PI-9 produced in P. pastoris.
Clone:: 7D8
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: PI-9 (proteinase inhibitor 9), also known as SerpinB9, is a 42kD intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:100 Immunohistochemistry (paraffin): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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