S1000-77X

SERPINB8 (Serine/Cysteine Proteinase Inhibitor, Clade B, Member 8, NK10, PI8)

Size

£825.00

SKU:: S1000-77X
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Ala24-Thr417 from human Serpin A9 expressed in NSO cells.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Serpin A9 (Serine proteinase inhibitor-clade A #9; also GCET1, Centerin and Serpin A11) is a 46 kD, secreted member of the a1-antitrypsin (clade A)-subfamily, serpin superfamily of protease inhibitors. It is only produced by normal and lymphoma germinal center B cells. Its function is unknown, but it presumably acts as an inhibitor of a trypsin-like protease. Mature human Serpin A9 is presumably 394aa in length. It contains one Serpin motif (aa405-415). There are multiple splice variants associated with Serpin A9. There are alternate start sites at Met19, Met99 and Met150. A fourth isoform shows a two aa substitution for (aa369-435), while a fifth isoform shares this same substitution coupled with a 39aa substitution for (aa1-75). A sixth variant shows a deletion of (aa91-190). Mature human Serpin A9 shares 67% aa identity with mouse Serpin A9. Applications: Suitable for use in ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 5-15ug/ml with immersion fixed paraffin-embedded sections of human lymphoma. Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with Recombinant Human Serpin A9. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice