S1000-77P2

SERPINB9 (Serine/Cysteine Proteinase Inhibitor Clade B Member 9, Serpin Peptidase Inhibitor Clade B (Ovalbumin) Member 9, Serpin B9, Cytoplasmic Antiproteinase 3, CAP3, CAP-3, Peptidase Inhibitor 9, Proteinase Inhibitor 9, PI9, PI-9)

Size

£709.00

SKU:: S1000-77P2
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant SERPINB9 produced in E. coli.
Clone:: Pi9-17
Format:: Supplied as a liquid in 0.7% BSA, 0.09% sodium azide.
Extra Details:: Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Not suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: Detects native SERPINB9 at 4~2kD as well as SERPINB9 complexed to granzyme B at ~67kD. As a positive control for SERPINB9 , YT-Indy cells (a natural killer leukemia cell line) can be used. In other cell lines a non-specific protein band of ~63kD can be detected. Immunohistochemistry: 1:100 Can be used on formaldehyde-fixed paraffin-embedded human tissues after pretreatment with 10mM citric acid pH 6.0 for 20 min. Immunocytochemistry: Fix cells for 10 min in 10% formalin and then treat them as paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.