S0668-55E
Semaphorin 4B (SEMA4B, Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain) (Biotin)
Size
£863.00
- SKU:
- S0668-55E
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Leu39-Glu712 from human Semaphorin 4B isoform 1, expressed in NS0 cells.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Extra Details:
- Semaphorin 4B (Sema4B; also SemaC) is a 95-100kD, class IV member of the Semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids in length. It contains a 674aa extracellular region (aa39-832) that is characterized by one Sema domain (aa65-502), a PSI region (aa520-574), and an Ig-like C2-type domain (aa599-658). There is one potential soluble splice variant that shows a 29aa substitution for aa707-832. Over aa39-712, human Sema4B shares 86% aa identity with mouse Sema4B. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml with recombinant human Semaphorin 4B Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice