Secretory Component (Biotin)

Size

£704.00

SKU:: S0630-01L
Purity:: Purified by DEAE chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified free monkey secretory component isolated from pooled milk.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labled with Biotin. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Extra Details:: Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Applications: Suitable for use in ELISA, Immunoelectrophoresis, Double Radial Immunodiffusion, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:500-1:1000 Immunohistochemistry: 1:20-1:100 Immunocytochemistry: 1:20-1:100 Optimal dilutions to be determined by the researcher. Biotin/IgG protein molar ratio (B/P): ~8.6 Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice