S0501-05
Sclerostin
Size
£754.00
- SKU:
- S0501-05
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 17aa at the C-terminus of human Sclerostin. Species Sequence Homology: rat; 94%, mouse; 88%.
- Format:
- Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS
- Extra Details:
- Sclerostin (213aa in rat and human (chr 17q12) and 211aa in mouse) is a novel secreted osteoclast-derived BMP antagonist with unique ligand specificity. It negatively regulates the formation of bone by repressing the differentiation and/or function of osteoblasts induced by BMPs. Since sclerostin expression is confined to the bone resorbing osteoclast, it provides a mechanism whereby bone apposition is inhibited in the vicinity of resorption; indicating the role of sclerostin in bone remodeling and links bone resorption and bone apposition. Defects in SOST are the cause of sclerosteosis, a progressive sclerosing bone dysplasia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml Western Blot: 1-10ug/ml using ECL technique. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: S0501-05A: Sclerostin, Human, Control Peptide. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice