S0495

Scl-70, Bovine, Rabbit (Scleroderma, DNA Topoisomerase I)

Size

£1,577.00

SKU:: S0495
Purity:: Purified using immobilized mouse monoclonal anti Scl-70. Purity is verified by SDS-PAGE. Other antigens (Sm, RNP, SSA, Jo-1 and SSB) are not detected with ELISA assays.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a frozen liquid in 0.01M Tris, 0.5M sodium chloride, 0.02% sodium azide.
Extra Details:: The Scl-70 antigen is an abundant nuclear protein susceptible to proteolysis (2). It has been shown that the Scl-70 antigen can be obtained as an extractable immunoreactive fragment (70 kD) of a larger protein (100-105kD), which has been shown to be Topoisomerase I (3). Anti-Scl-70 antibodies were originally described as precipitating autoantibodies in sera of patients with a connective tissue disease. It was later realized that some patients with diffuse scleroderma characteristically have a high incidence of this autoantibody (1). The presence of autoantibodies against the Scl-70 antigen has been reported to have prognostic value in the diagnosis of diffuse scleroderma, also termed Progressive Systemic Sclerosis (1). It has also been reported that autoantibodies to Scl-70 are a specific serologic marker for Systemic Sclerosis (3), and present in high proportions of Acrosclerosis (4). Anti-Scl-70 is not usually present in other autoimmune rheumatic diseases unless some cutaneous symptoms of scleroderma are present (4). Activity: 1000u/ml Unit Definition: One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for enzyme immunoassay. Activity will vary with assay conditions. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by researcher. Storage and Stability: Aliquot and store at -70+/-10°C. Aliquots are stable for 6 months after receipt at 70+/-10°C. Avoid freeze-thaw cycles. Note: Product is unstable above 0°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice