S0401-08

SCF (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Size

£544.00

SKU:: S0401-08
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to mouse Stem Cell Factor (SCF), expressed in E. coli
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O. For long term storage the addition of 0.09% sodium azide is recommended.
Extra Details:: SCF is a stromal cell derived cytokine that synergizes with other hemapoietic growth factors to stimulate the proliferation of myeloid and lymphoid progenitors in bone marrow culture. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml. (100ul/well) will detect at least 2000-4000pg/well of recombinant mouse SCF when used with S0401-08 as the detection antibody. Western Blot: 0.1-0.2ug/ml. Detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.