S0400-07V
SCF (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, Steel Factor, SF)
Size
£629.00
- SKU:
- S0400-07V
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant human stem cell factor, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
- Extra Details:
- SCF is a type I transmembrane protein and hematopoietic growth factor, which acts in synergy with other growth factors and signals through the c-Kit receptor. Interaction between SCF and the receptor tyrosine kinase c-Kit is an essential step for the proliferation, survival and differentiation of hematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. Studies have shown involvement of the association between SCF and c-Kit with the progression and invasiveness of tumors, and deficiencies of either SCF or c-Kit result in mast cell deficiency and macrocytic anaemia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml using 100ul/well. The detection limit is 0.2-0.4ng/well of recombinant human SCF Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human SCF is 1.5-3ng/lane under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice