S0160
Sarcoglycan, delta
Size
£843.00
- SKU:
- S0160
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Met1-Glu232 from human delta Sarcoglycan isoform 1, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 5% trehalose. Reconstitute with 500ul PBS.
- Extra Details:
- Delta-sarcoglycan is one of six known sarcoglycans, which are type I (alpha and epsilon) or type II (beta, gamma, delta or zeta) transmembrane glycoproteins. Sarcoglycans associate in different combinations in a tissue-specific manner, to form heterotetrameric, pentameric and hexameric membrane glycoprotein complexes (SGC). SGC interacts laterally with the dystroglycan complex, and both complexes are components of the dystrophin-associated glycoprotein complex (DGC), which forms a link between the cytoskeleton and the extracellular matrix. Mutations in sarcoglycans are associated with recessive autosomally inherited muscular dystrophy. Applications: Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml Immunohistochemistry: 10ug/ml; Detects delta-Sarcoglycan in human fetal heart tissue sections. Sections were fixed with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted S0160 overnight at 4°C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for 1 hr. Between each step, cells were washed with PBS, 1% BSA. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice