SARS E Protein (Severe Acute Respiratory Syndrome)

Size

£655.00

SKU:: S0097-98
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Raised against a synthetic peptide corresponding to amino acids at the carboxy-terminus of the SARS E protein (Genbank accession no. P59637)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Extra Details:: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers (4), which may explain its role in viral budding and virion envelope morphogenesis (5). Applications: Can be used for the detection of SARS E protein in ELISA. It will detect 10 ng of free peptide at 1 mg/ml. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.