S0094

SAP18 (SIN3-Associated Protein, p18)

Size

£745.00

SKU:: S0094
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human transcriptional repressor SAP18 (18kD)
Clone:: 6G10/H1
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: SAP18, also known as Sin3A-associated protein, which includes Sin3 (protein with four putative paired amphipathic helix domains), HDCA1 and HDCA2, is component of the histone deacetylase complex that plays an important role in the regulation of eukaryotic gene expression. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. It also has been shown to play a key role in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid. Applications: Suitable for use in ELISA and Western blot analysis. Recommended Dilution: ELISA: 1:8,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.