S0052-11K

S100 Protein, beta

Size

£384.00

SKU:: S0052-11K
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified bovine S100-beta protein.
Clone:: 3H47
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca++ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. S100 protein or Ca-binding protein A1/S100A1 is also a member of low mol wt, cytoplasmic, calcium binding troponin family. It is produced in the brain, muscle, heart and a wide variety of normal and tumor cells. S100 has two subunits: S100-alpha (mouse/rat/human 94aa, chromosome 1q21) and S100-beta (mouse, rat/human 92aa chromosome 21q22.2-q22.3) that forms either homodimer (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) and heterodimers (known as S-100a) of ~21kD. S100 alpha and beta chains show ~58% sequence identity. At least 9 different genes for S100 have been assigned on chromosome 21 namely S100A1-S1009. S-100 contains 2 EF-hand domains. S100A1 improves cardiac contractile performance both by regulating sarcoplasmic reticulum Ca ion handling myofbirilar Ca-ion responsiveness. Leakage of S100 protein by glial cells into the extracellualr matrix, CSF and serum are useful in monitoring traumatic brain injury. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000-1:5000 (ECL). S100-beta protein is ~21kD. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. In ELISA, antibody reactivity is independent of Ca-ion. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice