S100 Protein

Size

£754.00

SKU:: S0052-10A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Purified bovine S100 protein (containing both alpha and beta chains).
Format:: Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Extra Details:: Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. S100 protein or Ca-binding protein A1/S100A1 is also a member of low mol wt, cytoplasmic, calcium binding troponin family. It is produced in the brain, muscle, and heart and a wide variety of normal and tumor cells. S100 has two subunits: S100-alpha (mouse/rat/human 94 aa, chromosome 1q21) and S100-beta (mouse, rat/human 92 aa chromosome 21q22.2-q22.3) that forms either homodimer (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) and heterodimers (known as S-100a) of ~21kD. S100 alpha and beta chains show ~58% sequence identity. 9 different genes for S100 have been assigned on chromosome 21 namely S100A1-S1009. S-100 contains 2 EF-hand domains. S100A1 improves cardiac contractile performance both by regulating sarcoplasmic reticulum Ca ion handling myofbirilar Ca-ion responsiveness. Leakage of S100 protein by glial cells into the extracellualr matrix, CSF and serum are useful in monitoring traumatic brain injury. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000-5000 ECL. S100 protein (bovine and human) is ~21kD. ELISA: 1:10,000-1:100,000 Immunohistochemistry/Immunofluorescence: 1:50-1:100 in indirect staining of formalin-fixed, paraffin embedded tissue section. Optimal dilutions to be determined by the researcher. Positive Control for Western Blot (Human): S0052-10B Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice