S0051-85M

S100A7 (Protein S100-A7, Psoriasin, S100 Calcium-binding Protein A7, PSOR1, S100A7C)

Size

£825.00

SKU:: S0051-85M
Application:: IHC-Fr
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Met1-Gln101 from human S100A7 expressed in E. coli
Clone:: 11n168
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: S100A7 is an 11-13kD member of the S101 family, EF-hand superfamily of Ca-binding proteins. It is produced by keratinocytes and exists as both an intracellular and extracellular polypeptide. Intercellularly, it binds to E/FABP and may participate in fatty acid metabolism. Extracellularly, it is both monomeric and heterooligomeric and exhibits antibacterial properties. Human S100A7 is 101aa in length. It contains two EF-hand motifs aa13-48, 50-85, three zinc-binding His residues, one high-affinity Ca-binding site aa63-74 and an overlapping antibacterial sequence aa35-80. There appear to be no meaningful rodent structural orthologs. Human S100A7 is 93aa identical to human S100A15/7A, and the S100A7 gene has a duplication (S100A7B) that is 54aa identical to S100A7. Applications: Suitable for use in immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of human skin (hair follicle). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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