S0051-80Q

S100 Calcium Binding Protein A3, Recombinant, Human, aa1-101, His-Tag (S100A3, S100E)

Size

£512.00

SKU:: S0051-80Q
Molecular Weight:: 13.9
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 2mM DTT, 0.2M sodium chloride, 20% glycerol.
Extra Details:: S100A3 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A3 binds calcium with low affinity and zinc with high affinity and is believed to play a role in the calcium-dependent differentiation of cuticle cells and in the formation of hair shafts. Purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa1-101 of human S100A3, fused to His-tag at N-terminus, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF RECDYNKFMS VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS Q Molecular Weight: ~13.9kD (121aa), confirmed by MALDI-TOF Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.