S0050-01
S1 Nuclease, A. orzyae, ss-DNA degradation, Solution
Size
£332.00
- SKU:
- S0050-01
- Molecular Weight:
- 34
- Purity:
- Chromatographically purified.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- CAS Number:
- 37288-25-8
- Format:
- Supplied as a liquid in 30mM sodium acetate, pH 4.6, 50mM sodium chloride, 1mM zinc chloride, 50% glycerol.
- Extra Details:
- S1 Nuclease degrades single-stranded DNA and RNA yielding 5’-phosphoryl-terminated products. Applications: • Removal of protruding single-stranded termini in double-stranded DNA. • Selective cleavage of single-stranded DNA • Mapping RNA transcripts Quality Control: No double stranded DNA digestion was detected after incubation of 10units of S0050-01 with 1ug pUC 19 plasmid DNA for 1hour at 37°C. Unit Definition: One unit hydrolyses 1ug of denatured calf thymus DNA per minute at 37ºC, pH 4.6. Inhibitors: EDTA, citrate and high concentrations of SDS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice