R9951-50

Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK)

Size

£856.00

SKU:: R9951-50
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: NS0-derived, recombinant mouse Ryk, extracellular domain (aa35-211, UniProt #Q01887).
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Ryk (related to tyrosine (Y) kinase; also VIK and MRK) is a member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Mouse Ryk is 594 amino acids (aa) in length. It contains a 177 aa extracellular region (aa 35-211) that shows one WIF-1-like domain (aa 51-180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318-581). There is one alternate start site at Met117. Over aa 35-211, mouse Ryk is 94% aa identical to human Ryk, and shows absolute (100%) aa identity to rat Ryk. Applications: Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well. Immunocytochemistry: 10ug/ml. NIH3T3 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted R9951-50 followed by NL557-coupled anti-sheep IgG in the dark. Between each step, cells were washed with PBS containing BSA. Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice