R9950-05
Ryanodine Receptor
Size
£931.00
- SKU:
- R9950-05
- Purity:
- Purified by Protein G affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Canine cardiac ryanodine receptor. Cellular Localization: Membrane; Multi-pass membrane protein
- Clone:
- 9C106 (C3-33)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide
- Extra Details:
- The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus). Applications: Suitable for use in Western Blot Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detects a 565kD protein representing Ryanodine Receptor. In non-mammalian vertebrates a double is seen at 565kD representing the alpha and beta isoforms of the receptor. Immunohistochemistry (Frozen): 1ug/ml. Stains the myofiber consistent with the sarcoplasmic reticulum of the RyR IImmunofluorescence (IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice