R9915-20C
RUNX3 (CBFA3, AML-2, PEBP2-αC)
Size
£903.00
- SKU:
- R9915-20C
- Purity:
- Purified by Prroein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E. coli-derived rhRUNX3 aa186-415; Accession# Q13761).
- Clone:
- 10F614
- Format:
- Supplied as a lyophilized in PBS, 5% trehalose.
- Extra Details:
- RUNX3, also called CBFA3, AML-2 or PEBP2-αC, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-β. The runt domain (aa 54-186) is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shows 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile buffer. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice