RUNX2 (Runt-related Transcription Factor 2, Acute Myeloid Leukemia 3 Protein, AML3, CCD, CCD1, Core-binding Factor Subunit alpha-1, CBF-alpha-1, CBFA1, Oncogene AML-3, Osteoblast-specific Transcription Factor 2, OSF2, OSF-2, Polyomavirus Enhancer-binding Protein 2 alpha A Subunit, PEA2-alpha A, PEA2aA, PEBP2-alpha A, PEBP2A, PEBP2aA, PEBP2aA1, PEBP2A1, PEBP2A2, SL3/AKV Core-binding Factor alpha A Subunit)

Size

£594.00

SKU:: R9915-11
Application:: WB
Purity:: Purified by Protein G affinity Chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to somewhere between amino acids 250-300 from human RUNX2. Species sequence homology: rat and canine
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Extra Details:: RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Saos-2 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice