R9417-53
RSPO-4 (R-Spondin 4, Cristin-4)
Size
£748.00
- SKU:
- R9417-53
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Tyr21-Pro197 of mouse RSPO-4 expressed in E. coli.
- Clone:
- 8H192
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- R-Spondin 4 (RSPO-4) also cristin-4, is a heparin-binding, approximately 33kD, secreted member of the R-Spondin family. It is found in limb bud mesenchyme and developing heart. RSPO-4 reportedly binds the Wnt receptors Fzd8 and LRP6, either as a complex or as individual receptors. Mature mouse RSPO-4 is 209 amino acids (aa) in length. It contains a furin-like region (aa85-128), a TSP type-1 domain (aa138-197) and a basic aa rich region (aa199-221). There is one potential isoform where Arg substitutes for the C-terminal 82 amino acids. Over aa21-197, mouse RSPO-4 shares 98% and 85% aa sequence identity with rat and human RSPO-4, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml. Recombinant mouse R-Spondin 4 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice