RPA2, phosphorylated (T21) (Replication Protein A2)

Size

£780.00

SKU:: R3402-05C
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to human RPA2 T21 site. Species sequence homology: mouse (83%)
Format:: Supplied as a lyophilized powder in PBS, sodium azide, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Extra Details:: RPA2 is a 32kD DNA-binding protein that constitutess one of three subunits comprising the PRA heterotrimer complex. In conjunction with 70kD RPA1 and 14kD RPA3, RPA2 participates in DNA replication, recombination and repair. Human RPA2 is 270aa in length. It contains a Gly/Ser-rich N-terminus (aa1-33), a DNA-binding domain (aa43-171) and a protein-interaction C-terminus (aa187-270). Phosphorylation of the N-terminus on Ser4/8/23/29/33, plus Thr21, regulates RPA complex interactions with DNA repair and replication complexes. There are multiple splice variants. Three contain N-terminal extensions: one shows an 88aa insertion after Ser4, another shows a 12aa substitution for aa1-4, and a third shows a four aa insertion after Ser4. There is also a deletion of aa93-98, and a potential truncation after Gln175. Over aa141-270, human RPA2 shares 83% aa identity with mouse RPA2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml in lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line untreated (-) or treated (+) with 1uM Camptothecin (CPT) for 1 hour Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice