R3000-13
Rotavirus (FITC)
Size
£557.00
- SKU:
- R3000-13
- Application:
- IF
- Purity:
- Purified by chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Bovine (Nebraska Calf Diarrhea Virus).
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Rotavirus is a virus that infects the bowels and is the most common cause of gastroenteritis in infants. 7 major groups have currently been identified, 3 of which infect humans (groups A, B and C). Its genome consists of 11 segments of double stranded RNA held in the inner core of the three layered, icosahedral virion particle. The genome codes for 6 virus proteins (VP1,2,3,4,6,7) and 6 non-structural proteins (NSP1-6). Outer capsid proteins are VP4 and VP7, inner capsid -VP6 and core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. Applications: Suitable for use in Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Optimal dilutions to be determined by the researcher. Titer: Indirect Immunofluorescence: ~1:250 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice