R2039-22R

RNA Polymerase II, CTD Repeat (YSPTSPS) (RNA Pol II CTD, RNA Pol II-CTD, DNA-directed RNA Polymerase II Largest Subunit, RPB1, Pol2)

Size

£620.00

SKU:: R2039-22R
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5 YSPTSpPS (Human).
Clone:: 4H8
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added.
Extra Details:: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, ELISA, Western Blot, Flow Cytometry and Chromatin Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Chromatin Immunoprecipitation: 1:10-1:500 Flow Cytometry (Intracellular): 1ug/10e6 cells ELISA: 1:100-1:2000 Immunofluorescence (IC): 1:100-1:1000 Immunoprecipitation: 1:10-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.