R2032-24B

Rituximab

Size

£1,374.00

SKU:: R2032-24B
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: F(ab)2 fragment of Rituximab.
Clone:: 10F380 (MB2A4)
Format:: Supplied as a liquid in TBS, pH 8.0, 0.09% sodium azide.
Extra Details:: R2032-24B has been used to detect rituximab bound to the surface of the Raji B cell line, however detection of rituximab bound in vivo to B-CLL cells has not been demonstrated. It is possible that complement deposition on rituximab opsonised cells inhibits binding of the Anti-Rituximab Antibody to cell bound rituximab (Beum et al. 2004). Inhibition experiments carried out with Daudi cells demonstrated that this antibody is inhibitory at a ratio of 5:1 antibody:rituximab, but does not inhibit rituximab binding to CD20 at a ratio of 1:1. Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 50ug/ml; 10ul labels 10e6 cells in 100ul ELISA: 5ug/ml as coating antibody Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized rats were fused with cells of the NS-1 myeloma cell line. Recommended Negative Control: I1904-82D1: IgG2a, Rat Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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