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R2031

Ribonuclease T1, Aspergillus oryzae (RNase T1) (300Ku/mg)

Size

£320.00

SKU:
R2031
Molecular Weight:
11
Purity:
Chromatographically purified (SDS-PAGE)
Storage Conditions:
4°C
Supplier:
United States Biological
CAS Number:
9026-12-4
Format:
Supplied as a liquid in 2.8M ammonium sulfate.
Extra Details:
Ribonuclease T1 is an endonuclease which cleaves RNA or deaminated RNA between guanosine and inosine 3’-phosphate residues and the 5’-hydroxy residues of adjacent nucleotides with the formation of the corresponding intermediate 2’, 3’-cyclic phosphates. Quality Control: Certified free of contaminating non-specific endo-nuclease, exo-nuclease and ribonuclease Activity: ≥300,000 units/mg protein Protein Assay: As reported Unit Definition: One unit releases the equivalent of one unit A260 of acid-soluble products from yeast RNA in 15 minutes at 37°C at pH 7.5. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at 4°C. Aliquots are stable for 6 months after recepit at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice