R2031-74J

RIP3 (Receptor Interacting Protein 3, Receptor-interacting Protein 3, RIP-3, Receptor-interacting Serine/threonine Kinase 3, Receptor-interacting Serine/threonine-protein Kinase 3, RIP-like Protein Kinase 3, RIPK3)

Size

£626.00

SKU:: R2031-74J
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa497-518, QEGPKDPEAWSRPQ, of human RIP3.
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: RIP3 is a novel member of RIP Ser/Thr kinase family. It is a potent inducer of apoptosis and is an important component of TNFR1 signaling complex. RIP3 consists of an N-terminal RIP-like-Kinase domain and a unique, non-homologous C-terminus responsible for caspase activation and apoptosis induction. RIP3 is expressed in multiple tissues including hematopoietic cells and plays a functional role in the regulation of apoptosis and NF-kB signaling. It is recruited to the TNFR1 signaling complex in a RIP-dependent manner where it induces apoptosis by activating caspases and/or inhibiting TNFR1-induced NF-kB activation. RIP3 negatively regulates the TLR3-Trif−mediated NF-kB signaling pathway by competing with the binding of Trif to RIP1. The human RIP3 gene is localized in the chromosomal region 14q11.2. Applications: Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunohistochemistry (paraffin): 10ug/ml Immunofluorescence/iImmunocytochemistry: 1:20-1:500 Optimal dilutions to be determined by the researcher. Positive Control: NIH3T3 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice