R2031-62G-APC

Rictor (Rapamycin Insensitive Companion of mTOR, Rapamycin-insensitive Companion of mTOR, RICTR, AVO3, KIAA1999, Likely Ortholog of Mouse TORC2 Specific Protein AVO3 (S. cerevisiae), mAVO3, Pianissimo) (APC)

Size

£984.00

SKU:: R2031-62G-APC
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Rictor.
Clone:: 10F659 (9F1.2)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/g/uL. mTORC2 is activated by growth factors and other mitogenic signals. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: detects Rictor on 10ug of HEK293 cell lysates Immunoprecipitation: detects Rictor from 100ug of HEK293 cell lysates Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cell lysate. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.