R2011-19
Ribonuclease A (RNase A, Ribonuclease Pancreatic, RNase 1, RNASE1, RNS1)
Size
£448.00
- SKU:
- R2011-19
- Purity:
- Purified by ammonium sulphate precipitation and ion exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Ribonuclease A isolated and purified from bovine pancreas.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- Ribonuclease A is used to isolate DNA for RNA-free DNA. Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3’-Phosphate group of a pyrimidine nucleotide and the 5’-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains (Volkin and Cohn 1953). The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure was reported by Kartha, et al. (1967) and Lin, (1970). Wang, et al. (1975) indicate that flexibility in a three-dimensional structure is essential for optimal catalytic activity. Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid (Sekine et al. 1969). Applications: Suitable for use in Immunoelectrophoresis, RID, ELISA, Western Blot and Inhibition. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice