R1998-16

Rhodopsin (OPSD, Opsin 2, Opsin 2 Rod Pigment, Opsin-2, OPN2, Retinitis Pigmentosa 4, Retinitis Pigmentosa 4 Autosomal Dominant, RP4)

Size

£863.00

SKU:: R1998-16
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Membrane preparation from adult rat retina. Cellular Localization: Cytoplasmic.
Clone:: 2Q97 (RET-P1)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 1:50-1:200 for 20 minutes at RT. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Brain or retina Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.