R1998-12H

Rhodamine (TRITC, Tetramethylrhodamine Isothiocyanate Isomer R) (HRP)

Size

£704.00

SKU:: R1998-12H
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Highly purified Tetramethylrhodamine Isothiocyanate Isomer R (TRITC).
Clone:: NI 308
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 500ul sterile ddH2O. Dilute further in PBS, pH 7.2.
Extra Details:: The reactivity of the antiserum is directed to the TRITC molecule as tested in direct binding ELISA and immunoblotting. This antibody is intended to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. This finds particular application in a situation where there is weak monoclonal antibody binding to PBMCs expressing surface markers. This can also be applied to solid phase systems when used for the identification and/or quantitative determination of minute amounts of soluble specific antigens or antibodies. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Dot Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:100 Western Blot: 1:200 Immunohistochemistry (Paraffin): 1:10-1:40 Optimal dilutions to be determined by the researcher. Storage and Stability: Store lyophilized product at 4°C if to be used immediately within two weeks; store at -20°C for long-term storage. Upon reconstitution, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice