R1998-12
Rhodamine, Dihydro 123, Dihydrochloride Salt (Dihydrorhodamine)
Size
£393.00
- SKU:
- R1998-12
- Molecular Weight:
- 419
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- CAS Number:
- 109244-58-8
- Extra Details:
- Dihydrorhodamine 123 dihydrochloride is the HCl salt form of dihydrorhodamine 123 and is functionally equivalent to the latter. However, dihydrorhodamine 123 dihydrochloride is more water soluble and more stable to air oxidation than dihydrorhodamine 123. Thus, dihydrorhodamine 123 dihydrochloride is easier to use and can be stored longer than dihydrorhodamine 123. Dihydrorhodamine 123 (DHR 123) is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. It is not fluorescent until oxidized by ROS to the highly fluorescent product Rhodamine 123. Neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DHR, rather, these ROS are thought to combine with other cellular components such as cytochrome c oxidase or Fe2+ in order to oxidize DHR 123 to its fluorescent derivative Rhodamine 123. Formation of Rhodamine 123 can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1). Synonyms: D 23806; D 632; DHR CAS No: 109244-58-8 Molecular Formula: C₂₁H₁₈N₂O₃•2HCl Molecular Weight: 419 Purity: ≥95% (HPLC and TLC) Appearance: Off-white to pinkish solid Absorption/Emission: 505/534 nm (end product) Solubility: Soluble in DMF or Water at ≥10mg/ml. Do not use DMSO Storage and Stability: Powder is stable for 6 months after receipt at -20°C. Light sensitive.
- Shipping Conditions:
- Ambient