R1995-15A
RGS3 (Regulator of G protein signaling 3)
Size
£473.00
- SKU:
- R1995-15A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- E. coli-expressed human RGS3 (aa 401-519; corresponding to the C-terminal 119 residues)
- Format:
- Supplied as a lyophilized powder in 0.09% sodium azide.
- Extra Details:
- RGS3 (regulator of G protein signaling 3) is a 519-residue protein that is widely expressed. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase-activating protein (GAP). RGS3 negatively regulates the activation of Gi and Gq. A truncated RGS3 (RGS3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS3T may be localized in the nucleus and RGS3 is found in cytoplasm. Translocation to membrane is important for the RGS proteins to interact with heterotrimeric G proteins. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice