R1701-35C
Retinol Binding Protein 4 (RBP4, PRBP, PRO2222)
Size
£787.00
- SKU:
- R1701-35C
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Glu19-Leu201 of human RBP4, expressed in NS0 cells
- Clone:
- 7H193
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- RBP4, also known as plasma retinol-binding protein, is a member of the lipocalin superfamily that solubilizes and stabilizes retinol (vitamin A) for transport to the peripheral tissues. RBP4 shows retinol-dependent secretion by the liver, and its deficiency causes night blindness. In plasma, the 183 aa RBP4-retinol complexes with transthyretin (TTR), previously known as thyroxine-binding prealbumin, which prevents loss during glomerular filtration. Human and mouse RBP4 share 86% aa identity. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Flow Cytometry: 25ug/ml using HeG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeablized with methanol Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice