R1649-65-BIOTIN

Reticular Fibroblasts and Reticular Fibers (Fibroblast Marker) (Biotin)

Size

£1,081.00

SKU:: R1649-65-BIOTIN
Purity:: Purified by Protein G affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Isolated C3H thymic stromal cells
Clone:: ER-TR7
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: The antigen recognized by this clone has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:50-1:100 Optimal dilutions to be determined by the researcher. Hybridoma: P3-X63-Ag.653 myeloma cells with spleen cells from immunized rats. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.