R1637
Ret, phosphorylated (Tyr905)
Size
£639.00
- SKU:
- R1637
- Purity:
- Purified by Protein A and immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- The Ret proto-oncogene (c-Ret), a receptor tyrosine kinase, functions as a multicompetent receptor complex in conjunction with other membrane bound ligand-binding GDNF family receptors (aGFRs) (1). The ligands for c-Ret have been identified as the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (2-4). Gene alterations in c-Ret have been found to associate with different diseases including papillary thyroid carcinoma, multiple endocrine neoplasma type 2A (MEN2A), MEN2B, familial medullary thyroid carcinoma and a congenital developmental defect called Hirschsprungís disease (1,3). Tyr905 is located in the kinase domain of Ret and plays crucial roles in its catalytic and/or biological activities. Substitution of phenylalanine for Tyr905 dramatically inhibits its autophosphorylation activity (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice