Resistin-like Molecule, alpha (RELMa)

Size

£754.00

SKU:: R1587-04
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide corresponding to 14aa near the N-terminus of mouse RELM-alpha. conjugated to KLH.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Extra Details:: RELM-alpha/FIZZ1/PMNG1 (Parasite-induced Macrophage Novel Gene 1 (prepeptides: mouse/rat 111aa; ~77% identity) is 63% related to resistin at the C-terminus (38aa). The N-terminus of RELM-alpha is less conserved at the N-terminus. RELM-alpha mRNA was most abundant in white adipose tissue. It is also expressed in mammary tissue with significant fat pads. Unlike resistin, RELM-alpha is not expressed in 3T3-L1 adipocyte or in preadipocytes. It is also found in heart, lung and tongue, where resistin is absent. In mice, RELM-alpha/FIZZ1 is found at low levels in a subset of bronchial epithelial cells and in non-neuronal cells adjacent neurovascular bundles in the peribronchial stroma, and in the wall of the large and small bowel. FIZZ1 expression is induced hypertrophic, hyperplastic bronchial epithelium. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1-5ug/ml (ECL) ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Control Peptide: R1587-03: Resistin-like Molecule, alpha, Mouse, Control Peptide (RELMa) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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